extraction d or nikkari

extraction d or nikkari

Delta-6-desaturase gene polymorphism is associated with ...

2013-5-30 · Nikkari T, Luukkainen P, Pietinen P, Puska P: Fatty acid composition of serum lipid fractions in relation to gender and quality of dietary fat. Ann Med. 1995, 27: 491-498. 10.3109/07853899709002458. CAS Article PubMed Google Scholar 12.

Demander un devis

Employment of broad‐range 16S rRNA PCR to detect ...

2003-1-15 · Sensitivity of Qiagen Blood Extraction Kit for the extraction of genomic E. coli DNA from blood-EDTA samples. Lane M: DNA molecular weight marker (100 bp, Gibco, UK), lanes 1–9: DNA extracted from E. coli 10 9, 10 8, 10 7, 10 6, 10 5, 10 4, 10 3, 10 2, 10 1 CFU ml −1, respectively, lane 10: negative control (LAL grade water)

Demander un devis

Genetic Polymorphisms of Transcription Factor NRF2 and …

2016-4-10 · 8. Maatta KM, Nikkari ST, Kunnas TA. Genetic variant coding for iron regulatory protein HFE contributes to hypertension, the TAMRISK study. Medicine (Baltimore). 2015;94:e464 9. von Otter M, Landgren S, Nilsson S. et al. Association of Nrf2-encoding NFE2L2 haplotypes with Parkinson''s disease. BMC Med Genet. 2010;11:36-2350 -11-36 10.

Demander un devis

Evaluation of a protocol for molecular broad-range ...

2017-10-1 · DNA extraction DNA was extracted with the QIAamp DNA Mini Kit (Qiagen, Hilden, Germany). In order to enhance lysis of Gram-positive bacteria, protocols were slightly modified. For extraction of blood and other body fluids, an incuba-tion step with 10 000 units of the Ready-Lyse Lysozyme Solution (Epicenter, Madison, WI, USA) was included

Demander un devis

Arvottomat (1982)

1982-10-15 · Arvottomat: Directed by Mika Kaurismäki. With Matti Pellonpää, Pirkko Hämäläinen, Juuso Hirvikangas, Esko Nikkari. A criminal, his friend and his former …

Demander un devis

Real-Time Quantitative Broad-Range PCR Assay for …

Extraction protocol II resulted in no improvement in the sensitivity to detect S. aureus (Fig. (Fig.1C) 1C) or E. coli (Fig. (Fig.1D). 1D). By contrast, extraction protocol III allowed the detection of S. aureus and E. coli at concentrations as low as 10 1 CFU per PCR (Fig. 1E and F). These results suggested that extraction protocol III was ...

Demander un devis

Persistence of parvovirus B19 in synovial fluid and bone ...

Jackson DP, Lewis FA, Taylor GR, Boylston AW, Quirke P. Tissue extraction of DNA and RNA and analysis by the polymerase chain reaction. J Clin Pathol. 1990 Jun; 43 (6):499–504. [PMC free article] Nikkari S, Merilahti-Palo R, Saario R, Söderström KO, Granfors K, Skurnik M, Toivanen P. Yersinia-triggered reactive arthritis.

Demander un devis

Broad-Range Bacterial Detection and the Analysis of ...

2010-7-14 · In 1995, the Centers for Disease Control and Prevention (CDC) initiated a large-scale, population-based surveillance study to detect life-threatening infectious diseases in previously healthy persons 1 to 49 years of age; this study is known as the Unexplained Death and Critical Illness Project (UNEX) () rveillance was performed through the CDC Emerging Infections Program sites in …

Demander un devis

The extraction, quantification, and nature of hair lipid ...

1979-10-1 · The extraction, quantification, and nature of hair lipid The extraction, quantification, and nature of hair lipid SHAW, D. A. 1979-10-01 00:00:00 Synopsis A sensitive method is described for the spectrophotometric assay of hair lipid extracted from hair samples less than 10 mg in weight, i.e. for amounts of lipid down to about 5 μg. . The solvent‐free lipid extract is charred by heating in ...

Demander un devis

Evening primrose oil and olive oil in treatment of ...

The effects of 10 ml of evening primrose oil or olive oil, administered twice daily for 12 weeks, on clinical and laboratory signs and on plasma prostaglandins were studied in 18 patients with rheumatoid arthritis. The plasma concentration of PGE2 decreased and that of TxB2 increased in both treatment groups, but no significant improvement could be seen in either group.

Demander un devis

Comparison of four commercial DNA extraction kits ...

Comparison of four commercial DNA extraction kits for the recovery of Bacillus spp. spore DNA from spiked powder samples By Markos Molsa, Laura Kalin-Manttari, Elina Tonteri, Heidi Hemmila and …

Demander un devis

A MICROFLUIDIC CHIP COMBINING DNA EXTRACTION …

2011-8-25 · extraction, amplification, and detection of genomic DNA (gDNA) from bacteria found in saliva. Multiple species of bacteria, including Streptococcus mutans, Staphylococcus aureus, and methicillin-resistant S. aureus (MRSA), can be simultaneously identified through the use of parallel single-plex real-time polymerase-chain-reactions (rt-PCR).

Demander un devis

[PDF] Comparison of Commercial DNA Extraction Kits for ...

This study compares five commercially available kits for the extraction of bacterial genomic DNA from whole-blood samples and investigates the sensitivity, specificity, ease of automation, and overall efficiency of these methods. ABSTRACT The demand for molecular diagnostic tests in medical microbiology has highlighted the need for efficient methods of DNA extraction.

Demander un devis

Detection challenges in quantitative polymer analysis by ...

2020-9-16 · Accurate quantification of polymer distributions is one of the main challenges in polymer analysis by liquid chromatography. The response of contemporary detectors is typically influenced by compositional features such as molecular weight, chain composition, end groups, and branching.

Demander un devis

The lipids of bovine skin collagen

1972-3-1 · The lipid composition of "purified" bovine skin collagen was determined. Chloroform-methanol removed about 95 per cent of the lipids. The predominant lipids were triglycerides, cholesterol, cholesterol esters and some phosphatides. These lipids comprised about 13 per cent of the dry weight of the collagen preparation.

Demander un devis

Optimal DNA Isolation Method for Detection of Bacteria in ...

Lysis of bacterial cells. A total of 100 μl of S. pyogenes or H. influenzae cell suspensions (containing a total of 200,000 to 2 CFU) was transferred into sterile screw-cap Eppendorf tubes with 0.3 g of zirconia-silica beads (diameter, 0.1 mm; Biospec Products, Bartlesville, Okla.), and 100 μl of sterile UV-irradiated water (two times for 90 s each time with UV Stratalinker [Stratagene, La ...

Demander un devis

Comparative evaluation of automated and manual …

Automated DNA extraction methods can result in better real-time PCR detection levels than manual extraction kits, as factors like DNA purity and concentration can influence the sensitivity of real ...

Demander un devis

Preparation of Lipid Extracts Tissues

Extraction protocol II resulted in no improvement in the sensitivity to detect S. aureus (Fig. (Fig.1C) 1C) or E. coli (Fig. (Fig.1D). 1D). By contrast, extraction protocol III allowed the detection of S. aureus and E. coli at concentrations as low as 10 1 CFU per PCR (Fig. 1E and F). These results suggested that extraction protocol III was ...

Demander un devis

Frontiers | Isolation and Genetic Characterization of ...

Naïve Felidae in the wild may harbor infectious viruses of importance due to cross-species transmission between the domesticated animals or human–wildlife contact. However, limited information is available on virus shedding or viremia in the captive wild felids, especially in Malaysia. Four infectious viruses of , feline herpesvirus (FHV), feline calicivirus (FCV), canine distemper virus ...

Demander un devis

Tensor decomposition-based unsupervised feature …

2017-12-21 · Therefore, in this study, tensor decomposition (TD)-based unsupervised feature extraction (FE)—which is the extension of a recently proposed principal component analysis-based unsupervised FE that has been successfully applied to various bioinformatics problems [5–22]—was used for various gene expression profiles of murine tissues with ...

Demander un devis

Detecting the presence of bacterial DNA by PCR can be ...

2014-5-1 · DNA extraction and purification. For DNA extraction, 1 mL of body fluid or drainage was centrifuged at 10 000 g for 5 min, and total DNA was extracted from the pellet using a standard method. Briefly, the pellet was suspended in TE buffer containing 10% SDS to lyse the cells, and proteins were removed by digestion with proteinase K. Cell wall ...

Demander un devis

(PDF) Optimization of microwave-assisted extraction ...

Journal of Chromatography B, 825 (2005) 11–20 Optimization of microwave-assisted extraction followed by solid phase micro extraction and gas chromatography-mass spectrometry detection for the assay of some semi volatile organic pollutants in sebum Liz M. D´ıaz-V´azquez ∗, Oscar Garc´ıa, Zorangelys Vel´azquez, Ivelisse Marrero, Osvaldo Rosario Department of Chemistry, University of ...

Demander un devis

Cultural Isolation and Characteristics of the Blood ...

Background: On the analogy of the non-pathogenic microbiota found in oral cavity, skin and gastrointestinal tract, existence of blood microbiota was confirmed by DNA sequencing, but never deeply characterized. Hypothesis for the existence of dormant blood microbiota in healthy humans have been arisen and single species have been isolated. The aim of our study was to resuscitate and investigate ...

Demander un devis

Enzymatically oxidized phospholipids assume center stage ...

Lipids that are generated by enzymes in circulating blood cells through controlled oxidation of biomembranes have been discovered. These lipids, called enzymatically oxidized phospholipids (eoxPLs), are made when blood cells are activated, for example, during …

Demander un devis

Cultural Isolation and Characteristics of the Blood ...

2018-5-30 · Most of the DNA extraction kits: Tissue and cells genomic Prep kit and Blood genomic Prep kit, (GE, USA); Genomic DNA purification kit, (Thermo Fisher Scientific, USA); QIAamp DNA isolation kit, (Qiagen, Germany) and the stan-dard CTAB phenol/chlorophorm DNA …

Demander un devis

(PDF) Ligase chain reaction in detection of Chlamydia DNA ...

Many authors have detected chlamydia in the synovial fluid, synovial membrane or synovial tissue [5,8, 10, [14][15][16]. Nikkari et al. [10] observed C. trachomatis DNA in synovial fluid in 33.3% ...

Demander un devis

On the storage, transport, and extraction of serum lipids ...

1982-9-30 · The extraction with buffer (pH == 6), described in [4], was adopted after experi- mentation with a known [5] micromethod. These trials included adding 2.8 ml of a chloroform-methanol solution (2:1, v/v) to 200 jul pooled serum and shaking for 5 min with 0, 5, 20, 40, and 80 /xl of l mol/1 sulphuric acid or with 40 and 80 jul of water.

Demander un devis

Nous contacter